KMID : 0355620100360050341
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Journal of Korean Association of Oral and Maxillofacial Surgeons 2010 Volume.36 No. 5 p.341 ~ p.345
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Evaluation of osteogenic activity of periosteal-derived cells treated with inflammatory cytokines
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Park Bong-Wook
Choi Mun-Jeoung Hah Young-Sool Cho Hee-Young Kim Deok-Ryong Kim Uk-Kyu Kim Jong-Ryoul Byun June-Ho
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Abstract
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Introduction: Skeletal homeostasis is normally maintained by the stability between bone formation by osteoblasts and bone resorption by osteoclasts. However, the correlation between the inflammatory reaction and osteoblastic differentiation of cultured osteoprogenitor cells has not been fully investigated. This study examined the effects of inflammatory cytokines on the osteoblastic differentiation of cultured human periosteal-derived cells.
Materials and Methods: Periosteal-derived cells were obtained from the mandibular periosteum and introduced into the cell culture. After passage 3, the periosteal-derived cells were further cultured in an osteogenic induction Dulbecco¡¯s modified Eagle¡¯s medium (DMEM) medium containing dexamethasone, ascorbic acid, and ¥â-glycerophosphate. In this culture medium, tumor necrosis factor (TNF)-¥áwith different concentrations (0.1, 1, and 10 ng/mL) or interleukin (IL)-1¥âwith different concentrations (0.01, 0.1, and 1 ng/mL) were added.
Results: Both TNF-¥áand IL-1¥âstimulated alkaline phosphatase (ALP) expression in the periosteal-derived cells. TNF-¥áand IL-1¥âincreased the level of ALP expression in a dose-dependent manner. Both TNF-¥áand IL-1¥âalso increased the level of alizarin red S staining in a dose-dependent manner during osteoblastic differentiation of cultured human periosteal-derived cells.
Conclusion: These results suggest that inflammatory cytokines TNF-¥áand IL-1¥âcan stimulate the osteoblastic activity of cultured human periosteal-derived cells.
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KEYWORD
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Periosteal-derived cells, Inflammatory cytokine, Tumor necrosis factor-¥á(TNF-¥á), Interleukin-1¥â(IL-1¥â)
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